HiSeq : Flexibility for Multiple Applications
Among all next generation sequencers, Hiseq will be with no doubt the most widely used platform at the moment. Empowered by SBS(sequence by synthesis) chemistry, it generates the most amount of data per a run, making the price per Gb the lowest among all platforms. HiSeq has several variants depending on the amount of data they generate. DNA Link Sequencing Lab provides the services using HiSeq 4000 and 2500.
The HiSeq has become the undisputed powerhouse of Illumina sequencing systems for highthroughput sequencing applications. To date, millions of samples have been sequenced and thousands of papers have been published utilizing Illumina sequencing technology. The HiSeq has become the production platform of choice for all major Genome Centers and leading institutions around the world.
The HiSeq System delivers the highest daily throughput and total yield of any individually sold sequencer on the market today. With a massive daily throughput of 160 Gb per day or 1 Tb per run for HiSeq 2500, more samples can be simultaneously sequenced at a greater depth than ever before—producing richer, more meaningful data. Large studies can be completed with reduced hands-on time and reagent cost. In high output mode, the HiSeq 2500 System can process 8 human genomes at 30× coverage or 150 human exomes, assuming 4 Gb per exome at 2 × 75 bp, per run. The HiSeq 2500 System, in high-output mode, is perfect for production scale projects with large genome sizes or studies with hundreds of samples.
While the HiSeq System can generate massive data, it is also highly flexible. The HiSeq 2500 is the first Illumina sequencing platform to feature two run modes—rapid run and high-output run mode. Furthermore, the system can be configured to run one flow cell alone or two flow cells simultaneously—enabling a scalable solution tailored to your specific research needs. Rapid-run mode leverages on-board cluster generation and reduced cycle times to significantly accelerate run times. In rapid-run mode, fully automated clustering and sequencing for short read applications can be completed in 7 hours and a single human genome at 30× coverage can be completed in 27 hours. With rapid or high-output modes and single or dual flow cell options, the HiSeq 2500 can be tuned to meet output needs ranging from 10 Gb to 1 Tb or 300 million to 4 billion reads per run, supporting the broadest range of applications.
With the HiSeq 2500 System, high data quality is ensured by leveraging the most widely adopted chemistry in the industry: sequencing by synthesis (SBS). SBS technology on the HiSeq 2500 utilizes a proprietary reversible terminator-based method that detects single bases as they are incorporated into DNA template strands. As all four reversible, terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias and greatly reduces raw error rates compared to other technologies. The end result is highly accurate base-by-base sequencing that virtually eliminates sequence-context-specific errors, even within repetitive sequence regions and homopolymers. Illumina sequencing delivers the most accurate human genome, the highest yield of errorfree reads, and the highest percentage of base calls above Q30 in the industry
NextSeq : The First High-Throughput Benchtop Sequencer
The NextSeq Series delivers the power of high-throughput sequencing with the speed, simplicity, and affordability of a benchtop next-generation sequencing (NGS) system. The NextSeq 500 System fits seamlessly into research laboratories, without need for specialized equipment. Perform many combinations of high- and mid-throughput sequencing applications to advance your studies.
A transformative addition to the industry-leading Illumina next-generation sequencing (NGS) system portfolio, the NextSeq Series of systems delivers the power of high-throughput sequencing with the simplicity of a desktop sequencer. Its fast, integrated, sample-to-results workflow enables rapid sequencing of exomes, targeted panels, and transcriptomes in a single run, with the flexibility to switch to lower-throughput sequencing as needed.
The NextSeq Series enables researchers to keep pace with technology, putting them in control of their sequencing projects. These robust, scalable systems turn a broad range of high-throughput applications into affordable everyday research tools. The flexible NextSeq Series enables researchers to switch quickly from one application to another and configure output based on sample volume and coverage needs. Now, even the smallest laboratory can perform any combination of sequencing applications to advance their studies, supporting faster publication of landmark research.
Data are generated in as little as 12 hours for a 75-cycle sequencing run and less than 30 hours for paired 150-cycle reads. By employing the Illumina industry-leading sequencing by synthesis (SBS) chemistry and file format conventions, the NextSeq Series offers customers access to the broadest ecosystem of established protocols, workflows, data sets, and data analysis tools.
In DNA Link Sequencing Lab, we have multiple units of HiSeq and NextSeq units which are ready for serving researchers.
Which one to choose between HiSeq and NextSeq?
The practical difference will be the amount of data each machine generates. One flow cell of HiSeq consists of 8 lanes. Each lane is physically separated, and the user can apply different lanes with different samples. However, it is impossible to run each lane individually, meaning that we can only start sequencing when all 8 lanes are filled. Normally, sequencing companies fill the other lanes from other projects, so it is not a big problem. But there are some times when one or two lanes are left unloaded. This is where our Last minute sales shows up with which you can take advantage of lower price per lane.
On the other hand, NextSeq’s flowcell has two lanes, which are not physically separated, meaning that we cannot apply different samples (unless they are DNA barcoded.), and only one lane mix can be applied in the entire flowcell. Good thing is that the amount of data generated is much smaller than that of HiSeq, and it is easier to fill the flowcell. The price per Gb will be cheaper in HiSeq, but NextSeq gives more versatile usage for smaller projects.
The main technical difference between HiSeq and NextSeq will be the number of dyes each machines use. HiSeq uses traditional color coding with four different dyes, while NextSeq uses two dyes. This does not give any practical differences in terms of the data quality, but the trend in illumina sequencers are more into the direction of reducing the number of dyes.