Sequencing

 

Next Generation Sequencing (NGS) may be considered one of the biggest innovation in biotechnology history. It opened a huge opportunity to get close to the molecular mechanism about heredity, disease, and so on. Due to its powerful capability, diverse application are possible now.

DNA Link provides most of the sequencing applications. What makes DNA Link Sequencing Lab differs from other sequencing facilities is that we have all the major sequencing and genotyping platforms available, making it possible to provide a combined strategy.

Whole Genome Sequencing

Whole genome (re)sequencing is a method to analyze a specific polymorphism from a species with a known reference genome.  It provides the most comprehensive map of an organism’s genetic make-up. The method can discover molecular sarcoma and various genetic polymorphisms (SNPs, insertions, deletions, inversions, complex rearrangements, copy number variation) related to performance and other useful applications.

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Whole Exome Sequencing

Exome Sequencing is a method that selectively analyzes only coding region (Exon), which composes 1-2% of the genome. It is a cheaper and effective way of sequencing compared to whole genome sequencing that analyzes the entire genome.  The iterative detailing of the data amassed from exome sequencing can be very resourceful for teasing out, with high precision and reliability, single-nucleotide variants and de novo mutations associated with both Mendelian and common diseases. Based on these analysis results, exome sequencing can be effectively used in research for rare disease, cancer genomics, and genetic disorders.

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Whole Transcriptome Sequencing

Whole transcriptome sequencing is a major advance in the study of gene expression compared to traditional microarray-based approaches in that it provides a comprehensive view of a cellular transcriptional profile at a given biological moment enabling not only qualitative but also quantitative analysis of the transcriptome. It also provides information about diverse variations occurring at RNA level of post-transcriptional modifications such as splice variants and isoforms.

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Expression Profiling Sequencing

Taken apart the quantitative aspect of whole transcriptome sequencing, expression profiling sequencing gives most comprehensive insight about the transcription level of individual mRNAs which is then further related to the expression level of proteins. It is a very cost-effective way of understanding the transcriptions in that it only sequences a small part of mRNA (normally 3’ end with poly (A) tail) and counts the number of sequences. It provides virtually unlimited dynamic range for highly abundant transcripts as well as not missing rare transcripts.

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Isoform Sequencing

Empowered by the unique advantage of long reads from PacBio systems, isoform sequencing (Iso-Seq) reveals the splice variants of the transcripts providing the most comprehensive landscape of a transcriptome. The splicing junction of mRNA can be pin-pointed and this can be aligned back to the genome data to show what kind of splice variants are produced, further giving insights about the relation between splicing and a certain phenotype or diseases.

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Small RNA Sequencing

Small, non-coding RNA, or MicroRNAs are a short, 18-22 bp nucleotides which is known to play a critical role in the gene regulation and expression.

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ChIP-Seq, ATAC-Seq, DROP-Seq and so on..

Once you have your pre-treatment done with your samples, such as chromatin IP and so on, they are ready to be made into sequencing libraries in DNA Link Sequencing Lab. Just send the collected DNA and we will take care of them from there on. If you already have sequencing libraries on hand, that will be also ok. We will run them on our sequencer and deliver you with the sequence results.