Sample Submission Guide

PacBio Sequencers (Sequel / RSII)

Whole Genome de novo sequencing

Total of 15ug of gDNA with over 200ng/ul concentration is needed.

To maximize read length and quality, it is essential that your DNA sample:

  •  is double-stranded as single-stranded DNA is not compatible with the library preparation process.
  •  has not undergone multiple freeze-thaw cycles as they can lead to DNA damage.
  •  has not been exposed to high temperatures (e.g., >37 °C for 1 hour can cause a detectable decrease in sequence quality), pH extremes (< 6 or > 9). Air drying of pellets is preferred over heat drying.
  •  Avoid overdrying of genomic DNA. Do not heat when drying in a speed-vac.
  •  has an OD260/OD280 ratio of 1.8 to 2.0.
  •  does not contain insoluble material.
  •  does not contain RNA contamination.
  •  has not been exposed to intercalating fluorescent dyes or ultraviolet radiation.
  •  does not contain chelating agents (e.g., EDTA), divalent metal cations (e.g., Mg2+), denaturants (e.g., guanidinium salts, phenol), or detergents ( e.g., SDS, Triton-X100).
  •  does not contain carryover contamination from the starting organism/tissue (heme, humic acid, polyphenols, polysaccharides, etc.).
  • The concentration should be 200ng/ul or higher.
  • The amount of the DNA should be at least 15ug.


QC Criteria

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 15ug / (200ng/ul)
Nanodrop Purity 260/280 1.8~2.0
260/230  2.0 <
Agarose Gel Quality check No RNA contamination
No degradation
90% < 20kb


illumina Sequencers (HiSeq, NextSeq500)

 

Whole Genome resequencing

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 2-3ug / (20-40ng/ul)
Nanodrop Purity 260/280 1.8~2.0
260/230  2.0 <

Whole Exome sequencing

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 4ug / (25ng/ul)
Nanodrop Purity 260/280 1.8~2.0
260/230  2.0 <

RNA sequencing

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 2ug / (20-100ng/ul)
RIN RIN ≥ 7, No DNA contamination

Ion Torrent Sequencers (Ion Proton / S5)

 

Whole Exome sequencing

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 500ng / (10ng/ul)
Nanodrop Purity 260/280 1.8~2.0
260/230  2.0 <

Affymetrix

 

Axiom® Array

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 600ng / (10ng/ul)
Nanodrop Purity 260/280 1.8~2.0
260/230  2.0 <

 

GeneChip™ expression array

Detection platforms Criteria Standard value
Picogreen Amount/concentration total 1ug / (100ng/ul)
Nanodrop Purity 260/280 1.8~2.1
260/230  1.8~2.1
RIN RIN ≥ 7, No DNA contamination

Shipping Address

DNA Link operates several sample collection locations in the world. You may choose to send to the nearest shipping point.

Global Headquarters
150 Bugahyeon-ro
Seodaemun-gu
Seoul 03759
Republic of Korea

North America
DNA Link USA, Inc.
4901 Morena Blvd, Ste 703
San Diego, CA 92117
United States

Europe
DNA Link Europe
Fideliolaan 150
Amstelveen 1183PR
the Netherlands

Courier account number

You may choose to use your own courier account. If you do not have one, please contact us and we will give you our account number in Fedex, DHL or UPS. After sending your samples, please don’t forget to give us the tracking number. We will keep an eye on the route to ensure safe arrival of your samples.